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It did not seem likely that I was destined to undertake research on typhus.
Charles Jules Henry Nicole
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I was less successful in my attempts to effect preventive vaccination against typhus by using the virus and in trying to produce large quantities of serum using large animals.
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I finally demonstrated that typhus infection is not hereditary in the louse.
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I demonstrated the characteristics of experimental fever. It appears after an incubation period which is never less than five days. It follows the same pattern as natural fever in man, but is of shorter duration and less pronounced.
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Even if it had not been possible to reproduce the disease in animals and consequently to verify the hypothesis, this simple observation would have been sufficient to demonstrate the way in which the disease was propagated.
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From the practical point of view, the susceptibility to infection of the guinea pig proved to be the most useful step forward. Today, all laboratories use this animal for preserving the virus.
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At the time when I was conducting my research there was no known method for taking the guinea pig's temperature. I demonstrated a technique which is now widely used.
Charles Jules Henry Nicole
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