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I demonstrated the characteristics of experimental fever. It appears after an incubation period which is never less than five days. It follows the same pattern as natural fever in man, but is of shorter duration and less pronounced.
Charles Jules Henry Nicole
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Even if it had not been possible to reproduce the disease in animals and consequently to verify the hypothesis, this simple observation would have been sufficient to demonstrate the way in which the disease was propagated.

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From the practical point of view, the susceptibility to infection of the guinea pig proved to be the most useful step forward. Today, all laboratories use this animal for preserving the virus.

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